Comparison of Different Carbon Sources on Biomethane Production with Clostridium cellulovorans and Methanogens

Methane (CH4) has attracted attention as not only one of the hydrogen carriers in terms energy density, but also synthetic natural gas. In nature, decomposition organic compounds is performed with bacterial ecosystems that can produce CH4. Clostridium cellulovorans a decomposer was cultivated pig manure (PM) an unused biomass this study. As result high-performance liquid chromatography (HPLC) analysis, while formate and lactate were decreased C. medium containing 0.5% PM, acetate butyrate increased it. Accordingly, order to compare effect carbon sources for methane production, cocultivation methanogenesis Methanosarcina mazei or microbial flora production (MFMP) carried out medium. result, MFMP showed Moreover, comparison source either 1% methanol medium, after being precultivated glucose 12 h. The results revealed produced approximately eight times higher than After cultivation methanol, next-generation sequencing (NGS) analysis out. Interestingly, Methanofollis (0.211%), belonging methanogens through CO2 reduction pathway, dominant 72 h cultivation, siciliae (1.178%), M. barkeri (0.571%), (0.490%) major species cultivation. Since spp. belong acetoclasts (acetoclastic pathway), could promote growth spp., rather acetate. Therefore, it seems may play key role MFMP. Thus, these will provide important information low-cost biomethane production.